Table I. Summary of the complementation assay using wild-type and MLD point mutation versions of gDMI2-HAST.

While dmi2-1 plants transformed with wild-type gDMI2-HAST could generate a large number of pink nodules, roots expressing gDMI2-HAST versions with amino acid substitutions in MLD were impaired in nodule development and generated very few pink nodules. Control, Plants transformed with empty vector. Transformed roots were selected based on the mCherry fluorescence marker of the gDMI2-HAST construct. Experiments were repeated more than three times with similar results.

Parameter	Wild Type	C39D	Y95A	F111A	P164A	Y271A	F291A	Y294A	Control
Plants in total	13	14	11	12	13	14	12	11	14
Transformed plants	13	13	11	12	13	14	12	11	14
Plants with nodules	13	0	9	4	9	1	5	3	0
Nodule numbers	>300	0	29	4	17	1	19	4	0
Pink nodules	>300	0	5	0	0	1	2	0	0