Figure 2.

Expression of housekeeping and tissue marker genes measured by RNA-seq in microsamples. The seedlings were light-treated as described for Figure 1 and subjected to RNA-seq analysis. The RPM values are shown. White bar, without EOD-FR; gray bar, with EOD-FR. The inset in C is a magnified view of SUC2 expression in MN and VN. Six microsample pieces were combined to synthesize the cDNA (n = 2, mean ± sd). Asterisks indicate significant differences for Student’s t test (*, P < 0.05; ns, nonsignificant). Different letters denote significant differences between the FR-treated samples (P < 0.05, Student’s t test). A, Housekeeping genes; B, mesophyll marker genes; C, vasculature marker genes; D, epidermis marker genes.