Figure 4.

Confirmation of the RNA-seq results by RT-qPCR. Representative genes were selected from each of the six groups shown in Figure 3 and examined by RT-qPCR. The samples included the original samples for RNA-seq (n = 2) and freshly prepared samples (n = 3 or 4). The seedlings were light-treated as described in Figure 1. TUB2/TUB3 was used to standardize the results (mean ± sd). Asterisks indicate significant differences for Student’s t test (**, P < 0.005; *, P < 0.05; ns, nonsignificant). Different letters denote significant differences between the FR-treated samples (P < 0.05, Student’s t test). White bar, without EOD-FR; gray bar, with EOD-FR. The insets are magnified views of the expression in MN and VN, or VN and VS. RNA-seq results (Supplemental Table S2) with (solid line) and without EOD-FR (broken line) are shown for comparison. The expression levels are shown in arbitrary units setting the highest value to 1.