Figure 7.

Gene expression responses along the proximal-distal axis in the cotyledon. A, Diagram explaining the location of microsampling. The micro samples containing vasculature tissue were excised with the needle-based device for the RT-qPCR analysis. B to D, Relative expression of tissue marker genes (B), nonautonomous genes (C), and autonomous genes (D). The cDNA samples were prepared and analyzed as for Figure 1, except that six micro sample pieces were combined in a tube for cDNA synthesis. White bar, without EOD-FR; gray bar, with EOD-FR. TUB2/TUB3 was used to standardize the results. The expression levels are shown in arbitrary units setting the highest value to 1 (n ≥ 3, mean ± sd). Asterisks indicate significant differences for Student’s t test (**, P < 0.005; *, P < 0.05; ns, nonsignificant). Different letters denote significant differences between the FR-treated samples (P < 0.05, Student’s t test).