Average normalized nuclear count for each ploidy level detected in M. crystallinum juvenile plants. A, Seed. B, Cotyledon. C, First leaf pair. D, Second leaf pair. E, Third leaf pair. F, Fourth leaf pair. G, Fifth leaf pair. H, Roots. Leaves were measured over a 3-week period, as follows: week 1 (light gray bars), week 2 (medium gray bars), and week 3 (dark gray bars). Data are means ± se of three independent biological replicates.