Table I. Estimation of ploidy and nuclear and cell size in the EBC from leaves, stems, and flower buds.
Tissue | Treatment | Mean Nuclear Area | Nuclear Volume | No. | Estimated Ploidy from Fluorescence Microscopy | Flow Cytometry Size Estimate of Ploidy | Bladder Cell Area |
---|---|---|---|---|---|---|---|
µm2 | µm3 | µm2 | |||||
Bract leaf lower epidermis EBC | Water | 925 ± 79 | 117,842 | 21 | 1,024C | 128C | 28,577 ± 629 |
Salt | 1,815 ± 33 | 323,894 | 133 | 2,048C | 256C | 36,818 ± 861 | |
Bract leaf upper epidermis EBC | Water | 956 ± 52 | 123,816 | 20 | 1,024C | 128C | 18,641 ± 342 |
Salt | 978 ± 19 | 128,114 | 162 | 1,024C | 128C | 21,128 ± 454 | |
Flower bud EBC | Water | 1,230 ± 87 | 180,695 | 25 | 1,024C | 128C | 433,456 ± 15,707 |
Salt | 9,406 ± 154 | 3,821,167 | 35 | 32,768C | 65,536C | 2,208,355 ± 99,677 | |
Stem EBC | Water | 1,238 ± 46 | 182,461 | 26 | 1,024C | 128C | 42,111 ± 884 |
Salt | 6,586 ± 105 | 2,238,830 | 28 | 8,192C | 2,048C | 62,677 ± 1,969 |