Table I. Proteins abundant in the leaves of RNAi-SlMPK1 tomato line 1-24 compared with the wild type under HT conditions using iTRAQ.
UniProt Identifier | Gene Identifiera | Nameb | Ic | II | III | IV | ||||
---|---|---|---|---|---|---|---|---|---|---|
116:114d | Pd | 121:118 | P | 116:114 | P | 121:118 | P | |||
Q9M5A8 | Solyc07g042250 | CPN20 | 2.2284 | 0.00445 | 1.6144 | 0.04330 | 1.7567 | 0.08371 | 2.3335 | 0.02852 |
C5IU71 | Solyc05g052600 | SBPASE | 2.2080 | 4.37E-05 | 1.3183 | 0.00801 | 1.6749 | 0.51661 | 5.8076 | 7.05E-09 |
K4BAE6 | Solyc02g082760 | CAT2 | 1.9770 | 0.00157 | 3.1333 | 0.00022 | 1.3062 | 0.64461 | 1.8707 | 0.00136 |
K4B1S1 | Solyc01g103450 | CPHSC70-2 | 1.9767 | 3.24E-05 | 1.8880 | 0.01473 | 0.7379 | 0.09048 | 4.1687 | 6.57E-08 |
K4DAD5 | Solyc11g069790 | CPN60A | 1.8030 | 0.00306 | 2.7797 | 0.00334 | 1.5754 | 0.02498 | 1.8880 | 0.00038 |
K4CUF4 | Solyc09g065270 | RRF | 1.7539 | 0.00311 | 1.5276 | 0.02924 | 1.7311 | 0.50232 | 1.9770 | 0.04506 |
K4BW77 | Solyc05g005460 | NRX1 | 1.6444 | 0.03503 | 1.5704 | 0.03808 | 1.2022 | 0.04712 | 1.3062 | 0.160419 |
K4CH99 | Solyc07g064160 | THI1 | 1.6293 | 0.01993 | 4.6132 | 0.00785 | 0.9376 | 0.20589 | 1.9589 | 0.00549 |
K4BHA1 | Solyc03g063560 | GLU1 | 1.6144 | 6.86E-05 | 1.2942 | 0.04700 | 1.2803 | 0.95947 | 1.5704 | 1.68E-06 |
K4CEJ1 | Solyc07g043320 | 1.6144 | 0.00725 | 1.0186 | 0.655478 | 2.3768 | 0.00037 | 1.2474 | 0.02173 | |
Q672Q6 | Solyc02g079950 | PSBQ | 1.5276 | 0.00833 | 1.4723 | 0.00914 | 1.3908 | 0.06042 | 2.8054 | 0.00419 |
K4AYG3 | Solyc01g087730 | PRPL1 | 1.4860 | 0.01086 | 1.7865 | 0.00430 | 0.50583 | 0.01430 | 2.0512 | 0.02356 |
K4BW79 | Solyc05g005480 | AOR | 1.4859 | 0.00513 | 1.5996 | 0.083761 | 2.2909 | 0.00182 | 2.2699 | 0.03217 |
Q5NE20 | Solyc02g086820 | CA1 | 1.4454 | 0.01144 | 1.8197 | 0.03724 | 0.3162 | 0.01374 | 2.5586 | 0.00038 |
K4B1F9 | Solyc01g102310 | 1.4191 | 0.00155 | 1.1482 | 0.263537 | 1.6904 | 0.01809 | 1.5849 | 0.01383 | |
K4CQW8 | Solyc09g009390 | MDAR1 | 1.3062 | 0.03410 | 1.0765 | 0.993515 | 2.0137 | 0.03218 | 1.3727 | 0.01575 |
K4AV63 | Solyc01g028810 | CPN60B2 | 1.2942 | 0.00080 | 2.6062 | 1.04E-05 | 0.7178 | 0.46698 | 2.2080 | 0.00105 |
K4BX77 | Solyc05g009030 | IMD2 | 1.2706 | 0.02061 | 1.6293 | 0.03579 | 1.5560 | 0.99387 | 2.0324 | 0.02828 |
K4BMN4 | Solyc03g120850 | CPN60B1 | 1.2482 | 0.03575 | 2.2284 | 0.00645 | 1.0765 | 0.55860 | 3.0479 | 0.00764 |
K4B0D9 | Solyc01g097520 | ANNAT4 | 0.2089 | 0.00403 | 0.7516 | 0.488531 | 0.6546 | 0.04915 | 0.2378 | 0.00022 |
The UniProt identifier entries were obtained via UniProt (http://www.uniprot.org), and the gene identifiers were translated using UniProt’s ID Mapping. bThe name of the detected protein. cI and II indicate two biological replicates using an iTRAQ 8-plex, and III and IV indicate another two replicates in another iTRAQ 8-plex. dThese values were calculated as the ratio of 116 and 121 label to 114 and 118 label, respectively. 116 and 121 represent RNAi-SlMPK1 line 1-24 under HT, and 114 and 118 represent the wild type. Only proteins identified with more than one peptide and quantitation results with P < 0.05 were considered to be statistically different from unity. The proteins were considered to be differentially expressed if their iTRAQ ratios were greater than 1.2 or less than 0.67. The raw data are shown in Supplemental Table S3. Proteins with significantly different protein ratios (m/w) in at least three of four biological replicates are shown here. Significant changes are indicated by boldface type.