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. 2018 May 1;177(2):713–727. doi: 10.1104/pp.18.00017

Figure 5.

Figure 5.

OsFDML1 functions as heterodimers with its close paralog OsFDML2. A, The neighbor-joining phylogenetic tree of OsFDML1 with homologs in rice and Arabidopsis. The bootstrap values (%) based on 100 replicates are marked at the branching points. The scale bar indicates the number of nucleotide substitutions per site. OsFDML1 and the interacting protein OsFDML2 are marked in red. The red star indicates OsFDML1. The black stars indicate the other three paralogs OsFDML2, OsFDML3, and OsFDML4 in rice belonging to the same subgroup. B, OsFDML1 interacts with its close paralog OsFDML2 by yeast two-hybrid assay. C, OsFDML1 interacts with OsFDML2 in vivo by BiFC assay in rice protoplast. D, OsFDML1 localizes in nucleus and cytoplasm, and OsFDML2 localizes in nucleus in rice protoplast. Bars = 10 μm in C and D.