Figure 3.

Characterization of glycogen phosphorylase-deficient mutants (ΔglgP1, ΔglgP2, and ΔglgP1/2). A, Spot assay on solid BG₁₁ agar of the phosphorylase-deficient mutants to test resuscitation from long-term chlorosis. Dilutions are indicated on the top row. B, Determination of the glycogen content during resuscitation from 1-month chlorosis of ΔglgP2 and ΔglgP1/2. The glycogen content was normalized to 1 × 10⁸ cells. At least three biological replicates were measured. Error bars represent the sd. WT, Wild type.