Figure 5.

Characterization of glycolytic mutants ΔpfkB1/2, Δzwf, Δzwf/pfkB1/2, Δgnd, Δeda, and Δgnd/eda. A, Spot assay on solid BG₁₁ agar of glycolytic mutants to test resuscitation from long-term chlorosis. Dilutions are indicated in the top row. B, Determination of the glycogen content of glycolytic mutants during resuscitation from 1-month chlorosis of the same mutants. The glycogen content was normalized to 1 × 10⁸ cells. At least three biological replicates were measured; error bars represent the sd. WT, Wild type.