Figure 7.

Inducible knockout of OGT in skeletal muscle recapitulates the mKO mouse. OGT knockout was induced when mice were 5 months old by feeding doxycycline food for two weeks. Subsequently, mice were fed normal chow for 6 months before analysis. (A) Muscle from WT and imKO mice were immunoblotted with anti-OGT and -O-GlcNAc antibodies respectively. Equal amount of total protein was loaded across all the lanes. (B–F) Body weight (B), muscle mass (C), fat mass (D), fat depots (E), and energy expenditure (EE) (F) were assessed. (G–H) Glucose tolerance test (GTT) (G) and insulin tolerance test (ITT) (H). Data represent means ± SEM from n = 10 male mice in each genotype. *p < 0.05; **p < 0.01; ***p < 0.001 compared with WT control mice.