Figure 5.

Adipocyte Xbp1s overexpression protects mice from obesity. (A) Mice were maintained on regular chow until 27–32 weeks old to start Dox HFD feeding. Bodyweight of FIXs mice was reduced by Dox HFD and increased by regular HFD, which was not observed in WT mice (n = 7–11). The Chow group were age-matched WT mice that were maintained on regular chow during the course of study (n = 15). (B) Fat mass and lean mass of mice were determined by MRI after 100 days on HFD Dox (n = 6). (C)–(E) Mice on ob/ob background were maintained on regular chow until 11 weeks old to start Dox Chow feeding. Bodyweight mice were monitored for 166 days on Dox. Fat volume and muscle volume of mice were measured by CT scan at indicated time after switch to Dox chow (n = 3). (F) Mice were maintained on regular chow until 27–32 weeks old to start Dox HFD feeding. Bodyweight of mice was monitored after switch to Dox HFD, back to regular HFD and then Dox HFD again (n = 6). The Chow group were age-matched WT mice shared in the study of Figure 5A. (G) Mice were maintained on regular chow until 69 weeks old to start Dox HFD. Fat mass and lean mass of mice were determined by MRI 4 weeks after switch to HFD Dox (n = 8). Data were analyzed with two-tailed Student t test. *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001. Error bars denote SEM.