Figure 6.

Adipocyte Xbp1s overexpression increases fatty acid oxidation and heat production. (A) Xbp1s overexpression in adipocytes for 6 days increased fatty acid oxidation without affecting lipid uptake (n = 6). (B) Xbp1s induction in adipocytes for 90 days increased fatty acid oxidation in multiple fat depots without affecting lipid uptake (n = 6). (C) Body temperature was monitored after mice were switched to Dox chow (n = 4). (D) Xbp1s overexpression in adipocytes for 6 days reduced expression of most thermogenic genes in BAT (n = 8). (E)–(I) Metabolic cage studies were conducted to measure food intake, physical activities, O₂ consumption, CO₂ release, and heat production in mice. A 24 h fast was performed from 12 AM to 12 AM during the study (n = 5). Data were analyzed with two-tailed Student t test. *, p < 0.05, **, p < 0.01, ***, p < 0.001. ns, not significant. Error bars denote SEM.