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. 2018 Jun 7;9:758. doi: 10.3389/fpls.2018.00758

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Copyright © 2018 Liu, Ni, Zhang, Chen, Gao, Cai, Li, Sun and Qu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dehydration tolerance analysis of GhVHA-A in cotton. (A) Gene expression of GhVHA-A in silenced and control plant leaves by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. GhUBQ7 was used as a control. Experiments were repeated three times with similar results. (B) Time course of water loss in detached leaves from WT, EM and VIGS plants, expressed as a percentage of the initial fresh weight at indicated intervals. Values were means ± SE from three biological replicates with 10 plants per replicate (^∗P < 0.05; t-test).