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. 2018 May 23;145(10):dev162107. doi: 10.1242/dev.162107

Fig. 3.

Fig. 3.

Transfection efficiency analysis of Capsaspora. (A) Flow cytometry distribution of pONSY-Venus transfected cells. Area selected (P+) represents the Venus-positive population. (B) Flow cytometry distribution of pONSY-mCherry transfected cells. Area selected (P+) represents the mCherry-positive population. (C) Percentage of positive cells in single transfection. The box plot represents the transfection efficiency distribution over seven independent experiments with at least six technical replicates each (n=4.9 M cells). (D) Percentage of positive cells from a paired experiment with six technical replicates, transfecting either pONSY-Venus or pONSY-mCherry. Error bars represent s.d. (P=0.5625, Wilcoxon Signed Rank Test). (E) Wide-field fluorescence microscopy of a live cell co-transfected with pONSY-Venus and pONSY-mCherry. (F) Flow cytometry distribution of pONSY-Venus and pONSY-mCherry co-transfected cells. Cell population was divided into quartiles: negative cells (Q1), fluorescent cells expressing mCherry only (Q2), co-transfected cells expressing both fluorescent proteins (Q3), and fluorescent cells expressing Venus only (Q4). (G) Relative percentage of positive cells co-transfected with pONSY-Venus and pONSY-mCherry; expressing both fluorescent proteins (double), mCherry only or Venus only, calculated from the total number of positive cells in seven independent experiments with six replicates each (n=120,000 cells). Scale bar: 5 µm.