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. 2018 Mar 10;11:70–83. doi: 10.1016/j.molmet.2018.03.004

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

qPCR analysis of nutrient metabolite receptors on GLP-1 positive cells and 5-HT positive cells. (A) qPCR analysis expression data for main nutrient metabolite 7TM GPCRs in Glu-Venus positive cells (y-axis) compared with surrounding negative cells (x-axis) isolated from small intestine. (B and C) 5-HT positive (y-axis) and 5-HT negative (x-axis) cell gene mRNA expression from small intestine (B) and colon (C). The 45°-angled gray dotted lines display the fold change enrichment in 5-HT positive cells versus 5-HT negative cells and the gray-shaded square is marking what is considered noise.