Figure 6.

Impact of miR-31 mimic during differentiation in human adipocytes. (A) Oil red O staining of adipocytes in the culture dish of SGBS cells at the indicated days after induction of differentiation. (B) Percentage of differentiated adipocytes and glycerol levels during differentiation. (C) Expression levels of miR-31-5p and miR-31-3p in SGBS cells at indicated days after induction of differentiation (n = 3). (D) Expression of miR-31-5p at the indicated time points of differentiation after transfection with a miR-31-5p-specific mimic 2 days before treating SGBS cells with the differentiation cocktail. (E) mRNA expression of putative miR-31-5p targets at the indicated time points after differentiation. (*p < 0.05, **p < 0.01) Data are presented as ± SEM.