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. 2018 Mar 9;11:18–32. doi: 10.1016/j.molmet.2018.03.003

Figure 1.

Figure 1

Cyp2j4−/− mesenchymal stromal cells (MSCs) undergo spontaneous adipogenesis, and aging Cyp2j4−/− rats show increased body weight and larger adipocytes. (A) The genomic synteny between human CYP2J2 (reference) locus and the corresponding rat and mice loci. [ ] denote the interruption in the genomic distance scale; positions are in bp. (B) Oil-red-o (ORO) staining in MSCs from WT and Cyp2j4−/− before (left panel, pre-adipocytes) and after (right panel, adipocytes) adipocyte differentiation with the addition of adipogenic differentiation medium (DM). (C) ORO staining quantification as well as Fabp4 and Adipoq gene expression in MSC-derived pre-adipocytes and adipocytes. (D) Western blot analysis of PPARγ (pre-adipocytes) and C/EBPα (adipocytes) in Cyp2j4−/− and WT cells. Blots are representative of 2 independent experiments. (E) Western blot analysis of PPARγ and C/EBPα in the adipose tissue from WT and Cyp2j4−/− rats. (F) Glucose, insulin levels, and HOMA-IR index in 15-month old WT (n = 4) and Cyp2j4−/− (n = 6) rats' plasma (G) Body weight evolution in aging WT (n = 4) and Cyp2j4−/− (n = 6) rats under standard chow diet. (H) Representative Haematoxylin and Eosin (H&E) white adipose tissue (WAT) staining (left panel) and adipocyte area quantification in 15-month old WT and Cyp2j4−/− rats. Error bars are s.e.m. Scale bars; 250 μm (B); 100 μm (H).