Figure 2.

Cyp2j4^−/− rats show relatively increased metabolic dysfunction under CAF. (A) Percentage of body weight gain in WT (n = 5) Cyp2j4^−/− (n = 4) rats during 12 weeks of CAF. (B) Glucose, insulin, HOMA-IR, TAG, and NEFA levels measured in WT and Cyp2j4^−/− rats' plasma upon standard (STD) or cafeteria diet (CAF). At least n = 3 rats were used in each group. (C) Representative H&E staining in epididymal (left panel) and subcutaneous fat (right panel) sections. The arrows indicate the thickness of the subcutaneous adipose tissue layer and its larger magnification (×40) is shown at bottom left. (D) Adipocyte area distributions in WT CAF (open bars) and Cyp2j4^−/− CAF (black bars) where adipocytes are grouped into ascending sizes of 250 μm² (size range 250 μm²) in epididymal (left panel) and subcutaneous fat (right panel). For clarity, 9 group sizes are shown in the x-axis. Mean adipocyte area is shown for all groups (epididymal AT, top left). Subcutaneous (SBC) adipocyte layer and mean adipocyte layer are shown for WT (CAF) and Cyp2j4^−/− rats (subcutaneous AT, top right). (E) WAT cell density in WT and Cyp2j4^−/− rats in STD diet and CAF. At least n = 3 rats were used in each group. ns, non-significant. Scale bars, 100 μm (epididymal AT) and 500 μm (subcutaneous AT).