Skip to main content
. 2018 Mar 9;11:18–32. doi: 10.1016/j.molmet.2018.03.003

Figure 4.

Figure 4

De novo lipogenesis and increased gluconeogenesis in Cyp2j4−/− livers under CAF. (A) ORO staining and triglyceride (TAG) quantification in STD and CAF-treated WT and Cyp2j4−/− rats' livers. (B) ORO staining (left) and TAG quantification in 15-month old WT and Cyp2j4−/− livers. (C) C/EBPα Western blot analysis in STD and CAF-treated WT and Cyp2j4−/− rats' livers. Blots are representative of 2 independent experiments. (D) qRT-PCR analysis of Fasn in STD and CAF-treated WT and Cyp2j4−/− rats' livers. (E) LC-MS/MS heatmap displaying the proteins with significant differential protein abundance between Cyp2j4−/− (CAF) when compared with Cyp2j4−/− (STD) (150 and 164 up- and down-regulated proteins respectively, false discovery rate (FDR) < 0.05). In the heatmap, z-scores of the log-transformed intensities are displayed. Relevant functionally enriched pathways in these two protein sets are shown together with proteins contributing to these enrichments (red and blue bars). (F) LC-MS/MS heatmap (zoomed from E) and protein quantification profiles between WT (CAF) and Cyp2j4−/− (CAF) for Pgm1, Fbp1, Aldob, Ldha, Gapdh, Gpi, Pklr, Eno1, and Dlat. (G) Phospho-AKT (Ser473 and Thr308) and total Akt Western blot in CAF-treated WT (n = 3) and Cyp2j4−/−(n = 3) rats' livers. Numbers denote biological replicates. Error bars are s.e.m. At least n = 3 rats were used in each group. ns, non-significant. Scale bars, 100 μm.