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Chronic alcohol consumption reduces transcriptional expression of autophagy-related proteins in the liver. Relative mRNA expression of (A) Beclin-1, (B) ATG-5, (C) ATG-3 and (D) Bcl-2 was analyzed by real-time polymerase chain reaction. HPRT served as housekeeping gene, expression of control was set to 100%. *p<0.05; **p<0.01 compared with controls; data are presented as mean ± SEM (n=10). ATG=autophagy-related gene; Bcl-2= B-cell lymphoma 2 gene.
