Table 1.
gene | forward primer 5´- 3´ reverse primer 5´- 3´ |
Probe 5´ 6-FAM - TAMRA-3´ |
---|---|---|
ATG-3 | GCAGCACCATGCAGGTGAG TGGTCACTCGGTCCAGGATC | TCGTGTGCCAGCGCTGTAGCC A |
ATG-5 | ACATCAGCATTGTGCCCCA TGTCATGCTTCGGTGTCCTG |
CAGACTGAAGGCCGTGTCCTGCTCA |
Beclin-1 | TGCGACAGTCTCTCCGTGC GGCCACTTCCAGAGCCTTTC |
TGCTCCGGTCCCAGGATGCAGA |
Bcl-2 | TGAACCGGCATCTGCACATGAACCGGCATCTGCACA AGAGGTCGCATGCTGGG |
AACGGAGGCTGGGATGCCTTTGTG |
HPRT | GGAAAGAACGTCTTGATTGTTGAA CCAACACTTCGAGAGGTCCTTTT |
CTTTCCTTGGTCAAGCAGTACAGCCCC |
Abbreviations: qRT-PCR= quantitative real-time reverse transcriptase polymerase chain reaction; ATG= autophagy-related protein; Bcl-2=B cell lymphoma 2; HPRT=Hypoxanthine-guanine phosophoribosyltransferase