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Inhibition of neddylation by MLN4924 enhance ATRA induced autophagy. NB4 cells were treated with ATRA (0.01 μM) and/or MLN4924 (40 nM) for 24 h, and the expression of cullin3, cullin1, p27kip, DAPK1, Beclin1, p-Beclin1 and LC3 were analyzed by Western blot. The expression of β-actin was used as loading control.
