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Effect of Aneustat on LNCaP cells. LNCaP prostate cancer cells were treated with Aneustat. Effect of Aneustat (200 μg/ml) on (a) cell numbers at 24 hr and 48 hr; (b) glucose consumption and lactate secretion at 48 hr; (c and d) mRNA expression of genes of the aerobic glycolysis pathway; (e) protein expression of GLUT1, LDHA and MCT4. The experiments were carried out independently (3×). **p < 0.01; ***p < 0.001; NS: not significant.
