Figure 3.

Inhibitory effect of cGLE on glucose transport under sodium‐free conditions. Caco‐2 cells were grown on collagen‐coated 0.4 μm transwell inserts for monolayer formation and fast differentiation. On days 5–7, glucose transport across the cell monolayer was quantitated. HEPES buffer (sodium chloride was replaced with potassium chloride) with 2.1 g L⁻¹ glucose and 1.0 g L⁻¹ xylitol as well as the indicated extract (100 mg L⁻¹) was used as a donor solution in the apical compartment. Samples were collected from the basolateral compartment (HEPES buffer) at the respective time points. Glucose concentration of the samples was measured by HPLC. Error bars are based on the standard error of the mean (n = 10 inserts, measured on 5 different days).