Figure 3.

miR‐34b‐5p expression in whole lungs at different times after LPS injection in mice. (a) Quantitation of the miR‐34b‐5p levels in the lungs from mice with LPS injection at different times. The miR‐34b‐5p mimic or inhibitor and corresponding NC were transfected into RAW264.7 cells to validate the relationship between miR‐34b‐5p and PGRN. (b) Quantitation of the transfection efficiency of miR‐34b‐5p mimic. (c) Quantitation of transfection efficiency of miR‐34b‐5p inhibitor. (d,e) The effects of the miR‐34b‐5p mimic and inhibitor on the PGRN protein level in RAW264.7 cells were assessed by Western blot, and β‐actin was as a loading control. (f,g) The effects of the miR‐34b‐5p mimic or inhibitor on PGRN mRNA in RAW264.7 cells were assessed by RT‐PCR, and β‐actin was used as a control. (h) Quantitation of the dual‐luciferase reporter assay, n = 6. (i) Schematic of the WT PGRN 3′UTR and mutated PGRN 3′UTR luciferase constructs. *p < 0.05. Data are presented as the mean ± SD