Figure 6.

miR‐34b‐5p inhibition significantly decreases inflammatory mediator levels in the BALF and improves the survival ratio during ALI. Mice were subjected to LPS after administration of the miR‐34b‐5p antagomir or NC for 48 hr. (a) MPO in the lungs was assessed by ELISA. (b) The total number of BALF cells and neutrophils was determined with cytospin and a hemocytometer. (c) Lung W/D ratios. (d) The protein concentrations in the BALF were determined with BCA assays. The levels of (e) TNF‐α, (f) IL‐6, (g) IL‐1βin the BALF. (h) Mouse survival (n = 10) was monitored at different times for 48 hr. *p < 0.05. Data are presented as the mean ± SD