Figure 2.

Effect of miR‐130b on resistance to cisplatin in cisplatin‐resistant lung cell lines. (A) miR‐130b level in A549 and H446 parental cells and cisplatin‐resistant cells was detected by real‐time PCR. (B) A549/CR and H446/CR cells were transfected with miR‐130b inhibitor or inhibitor‐NC for 24 hours; miR‐130b level was detected by real‐time PCR. (C) A549/CR and H446/CR cells were transfected with miR‐130b inhibitor or inhibitor‐NC for 24 hours and then treated with increasing concentrations of cisplatin for 72 hours. Cell proliferation was determined by MTS assay. (D)A549/CR and H446/CR cells were transfected with miR‐130b inhibitor or inhibitor‐NC for 24 hours and then treated with 40 μM cisplatin for 24 hours. Apoptosis was analysed by Annexin V/PI staining followed by flow cytometry (left). Caspase 3/7 activity was determined by fluorogenic analysis (right). Result in (A), (B), (C), and (D) was expressed as means ± SD of 3 independent experiments