Figure 4.

PTEN is a direct target gene of miR‐130b in lung cancer. (A) The predicted binding site of miR‐130b in the 3′‐UTR of PTEN. (B) The relative luciferase activity of A549 cells was detected after PTEN‐3′‐UTR WT or Mut were co‐transfected with miR‐130b mimic and mimic‐NC. (C) A549 cells were transfected with miR‐130b mimic or mimic‐NC for 24 hours, PTEN mRNA was detected by real‐time PCR (left), and PTEN protein level was analysed by western blotting. (D) A549 cells were transfected with miR‐130b inhibitor or inhibitor‐NC for 24 hours, mRNA was detected by real‐time PCR (left), and PTEN protein level was analysed by western blotting