Figure 5.

miR‐130b promotes cisplatin resistance via Wnt/β‐catenin signalling pathway. (A) A549 cells transfected with miR‐130b mimic with or without PTEN plasmid co‐transfection, PTEN expression were analysed by western blotting. (B) A549 cells transfected with miR‐130b mimic with or without PTEN plasmid co‐transfection were treated with increasing concentrations of cisplatin for 72 hours. Cell proliferation was determined by MTS assay. (C) A549 cells were transfected with miR‐130b mimic or mimic‐NC for 24 hours, indicated protein level was analysed by western blotting. (D) A549 and A549/PTEN cells were transfected with miR‐130b mimic or mimic‐NC for 24 hours, indicated protein level was analysed by western blotting. (E) A549 and A549/PTEN cells were transfected with miR‐130b mimic or mimic‐NC for 24 hours and then treated with 40 μM of cisplatin for 24 hours