Figure 1.

Selective reduction in Rag2GFP⁻ Foxp3⁺ thymic T‐Reg in Aire^−/− mice. Flow cytometric analysis of CD4⁺CD8⁻TCRβ⁺CD25⁺Foxp3⁺, CD4⁺CD8⁻TCRβ⁺CD25⁺Foxp3⁻ and CD4⁺CD8⁻TCRβ⁺CD25⁻Foxp3⁺ thymocytes from both WTRag2GFP (white) and Aire^−/−Rag2GFP (black) mice. Gating strategy is shown in Supporting Information Fig. 1. Bar graphs in panel (A) show total numbers of each population, and panel (B) shows representative examples of Rag2GFP expression within each population, gray histograms indicated fluorescence levels on GFP⁻ thymocytes. Panel (C) shows numbers of Rag2GFP⁺ T‐Reg, CD25⁺Foxp3⁻ and CD25⁻Foxp3⁺ cells in WTRag2GFP (white) and Aire^−/−Rag2GFP (black) mice. Panel (D) shows numbers of Rag2GFP⁻ T‐Reg, CD25⁺Foxp3⁻ and CD25⁻Foxp3⁺ cells in WTRag2GFP (white) and Aire^−/−Rag2GFP (black) mice. Error bars indicate SEM, using an unpaired Student's two‐tailed t‐test: *p < 0.05; ***p < 0.001. Data are pooled from at least three separate experiments, with at least 6 mice of each strain in total.