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. 2018 Jan 29;48(5):844–854. doi: 10.1002/eji.201747375

Figure 5.

Figure 5

Aire is dispensable for post‐selection maturation and cytokine licencing in conventional thymocytes. Flow cytometric detection of CD4+CD8−TCRβ+CD25−Rag2GFP+ conventional CD4+ SP thymocytes in WTRag2GFP (A) and Aire−/−Rag2GFP (B) mice. Analysis of Qa2/CD69 expression in these cells is also shown. Panel (C) shows conventional CD4+ SP thymocytes from WTRag2GFP and Aire−/−Rag2GFP mice, analysed for expression of CD69 and MHCI to detect: SM (CD69+MHCIlow), M1 (CD69+MHCI+) and M2 (CD69−MHCI+) subsets. Histograms show the Rag2GFP levels in the indicated populations. Panel (D) shows proportions, numbers and Rag2GFP levels of populations identified in (C). Panels (E) and (F) show analysis of TNFα production in SM, M1 and M2 subsets of conventional CD4+ SP thymocytes from WTRag2GFP (white bars) and Aire−/−Rag2GFP (black bars) mice. Error bars indicate SEM, and a one‐way ANOVA was used, * p< 0.05. Data are pooled from at least three separate experiments, with at least 6 mice of each strain in total.