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CE‐MS separation of post‐translational modified synthetic peptides using a neutrally coated capillary. (A) Total ion current electropherogram and extracted ion electropherograms of (B) mono‐phosphorylated and (C) tri‐ and tetra‐phosphorylated peptides. CE conditions: Separation capillary length: 100 cm with porous tip, i.d.: 30 μm, o.d.: 150 μm; BGE: 10% (v/v) acetic acid; separation voltage: +30 kV with a pressure of 1 psi applied at the capillary inlet.
