Figure 3.

Flavonoids show cytoprotective effects on the hFOB 1.19 cells treated with zoledronate. A, The hFOB 1.19 cells were treated with galangin, icariside II, kaempferol, and quercetin (1‐50 µM) for 24‐72 h, and cell viability was assessed using an MTT assay, which was measured using an ELSIA reader. B, The hFOB 1.19 cells were pretreated with galangin (5 µM), icariside II (1 µM), kaempferol (5 µM), and quercetin (5 µM) for 24 h; after, they were treated with zoledronate (50 µM) for 48 h. The cells pretreated with flavonoids and treated with zoledronate showed higher viability than the cells treated with zoledronate alone. C, Western blot analysis showed that collagen I, osteocalcin, BMP‐2, ALP, Osterix, and RUNX2 increased in the cells pretreated with flavonoids and treated with zoledronate more than the cells treated with zoledronate alone