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Primers used in this study
| Gene | Forward primer (5′‐3′) | Reverse primer (5′‐3′) |
|---|---|---|
| Collagen I | TGAACTTGTTGCTGAGGGCA | ACTGGGCCAATGTCCACAAA |
| BMP‐2 | GCTGTCTTCTAGCGTTGCTG | CTGTTTCAGGCCGAACATGC |
| ALP | ATCTTCCTGGGTGACGGGAT | CATGGCCAGGAAGGTCTCAG |
| RUNX‐2 | GCGCATTCCTCATCCCAGTA | GGCTCAGGTAGGAGGGGTAA |
| Osterix | AAACCCAAGGCAGTGGGAAA | TGCCCCCATATCCACCACTA |
| LC3B | GGCCTTCTTCCTGTTGGTGA | TCTCCTGGGAGGCATAGACC |
| SQSTM1 | CATCGGAGGATCCGAGTGTG | TTCTTTTCCCTCCGTGCTCC |
| GAPDH | GTCAAGGCTGAGAACGGGAA | AAATGAGCCCCAGCCTTCTC |
