Skip to main content
. 2018 May 21;14(7):799–806. doi: 10.7150/ijbs.25928

Figure 3.

Figure 3

Blockade of IGF-I and IGF-II binding to receptors by eAds. (A) Inhibition of IGF-I or IGF-II binding to MCF-7 cells. Biotinylated IGF-I was preincubated with s7g1 and control m36 at concentrations of 10 nmol/L or 100 nmol/L. Then, mixtures were used to stain with MCF-7 cells. Cells were detected by streptavidin-PE. In the graphs, black lines are for cells without staining. Green and blue lines are for tested antibodies at 10 nmol/L and 100 nmol/L, separately. Red lines represent binding of IGF-I or IGF-II alone. (B) Inhibition of IGF-I or IGF-II binding to IGF-1R in ELISA. IGF-1R was directly coated on the ELISA plate. Serial dilutions of biotinylated IGF-I or IGF-II were added to ELISA plates in the presence of competitors (s7g1). Bound IGF-I or IGF-II was detected by HRP-conjugated streptavidin antibody. The reaction was read at 450 nm. Shown are data with mean ± SEM calculated from 3 separate experiments.