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. 2018 May 21;14(7):799–806. doi: 10.7150/ijbs.25928

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Competition of s7g1 with m708.5 binding to antigens. (A-B) competition ELISA. IGF-I (A) or IGF-II (B) was directly coated on the ELISA plate. Serial dilutions of s7g1 were added in the presence of competitors (IgG m708.5 or a control IgG). Bound s7g1 was detected by HRP-conjugated anti-Flag antibody. (C) Sandwich ELISA. IGF-II was captured with IgG m610 coated on the ELISA plate. Bound s7g1 and scFv m708.5 were detected by HRP-conjugated anti-FLAG antibody. Shown are data with mean ± SEM calculated from 3 separate experiments.