Figure 3.

Mineralization occurs along with odontoblast-processes. a. A schematic illustration of long interval injections of Calcein and Alizarin-red at -7 days and -2 days respectively (top left panel); the low magnification view of the cross section of incisor (top right panel) and the enlarged confocal view of numerous labeled odontoblast-processes with the green line on the top and red line at the bottom; and b. The confocal images of the 1^st molar labeled with Calcein at -20 hours and Alizarin Red at -4 hours revealed a clear pattern of the mineral deposited along odontoblast-processes by the enlarged sagittal view (lower left panel), and the cross view (lower right panel). Of note, the only difference of the labelled mineral collars between the surface and the inside dentin is the diameter and density of odontoblast processes. As a result of the merged mineral collars on the surface, the mineralization front is observed.