Figure 7.

High expressions of biglycan and nestin in predentin but not in dentin. a. The 2.3 Col with alizarin-red label (reflecting the mineralization event; left panel) and the Gli1-activated Td tomato (red color), in which the immuno-stained biglycan (green color) is restricted in the predentin (indicating an association with a lack of minerals in the predentin; right panel); and b. The Gli1-activated tomato molar slide, in which the immuno-stained nestin (green) is restricted in the odontoblast-process located in the predentin. Data indicate a likely role of these molecules in prevention of mineralization in predentin.