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. 2018 Jun 11;6:e4959. doi: 10.7717/peerj.4959

Figure 4. Representative images and semi-quantitative analysis of ECM mineralization at Day 14 of culture.

Figure 4

(A) Representative images of cells not treated (C), and treated with ES for three (D3), seven (D7), and 14 (D14) days, stained with Alizarin Red at Day 14 of culture. (B) Quantification of Alizarin Red stained calcium deposits. Amount of calcium deposits in D7 and D14 cells was significantly (p < 0.05) higher in comparison to control (C) and D3 cells. No difference was detected between the amount of calcium in D7 and D14 cells, or between C and D3 cells. Different letters on bars indicate significant differences among groups.