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(A) Alkaline Phosphatase (ALP) expression was measured in all groups at Day 14 of culture. No significant difference in ALP amounts was detected between the groups. (B) Expression of RunX2, (C) ColIa1, (D) Osteopontin, (E) Osterix and (F) Calmodulin genes were measured by mean of qRT-PCR at Day 3, 7, and 14 of culture. Asterisk indicates significant (p < 0.05) differences among groups within the same time point.
