Table 2. qRT-PCR primers sequences.
| Gene | Forward primer | Reverse primer |
|---|---|---|
| Calmodulin | TTTGACAAGGATGGCAATGGCT | TGTTAGCTTTTCCCCGAGGT |
| ColIa2 | TTCCCGGTGAATTCGGTCT | ACCTCGGATTCCAATAGGACCAG |
| Osteopontin | GATGAACAGTATCCCGATGCC | TCCAGCTGACTTGACTCATGG |
| Osterix | CTGGGAAAAGGAGGCACAAAG | GGGTGGGTAGTCATTGGCATAG |
| Rplp1 | GCATCTACTCCGCCCTCATC | GCATCTACTCCGCCCTCATC |
| RunX2* | * | * |
| Ywhaz | GATGAAGCCATTGCTGAACTTG | GTCTCCTTGGGTATCCGATGTC |
Notes.
ColIa2, collagen type I alpha 2 chain; Rplp1, Ribosomal protein P1; Ywhaz, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta; * RunX2, Runt-related transcription factor 2 purchased from Qiagen (330523; Qiagen, Hilden, Germany).