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. 2018 May 23;8:169. doi: 10.3389/fcimb.2018.00169

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Copyright © 2018 Bondu, Bitting, Poland, Hanson, Harkins, Lathrop, Nolte, Lawrence and Buranda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistologic examination of lobar pneumonia patient (case P6 Block B) shows strong staining for neutrophil elastase, but poor staining for P2Y₂R and uPA. (A) Micrograph of lung tissue stained with hematoxylin and eosin (H&E) showing numerous neutrophils congest alveolar space and relatively few macrophages and lymphocytes. (B) Intense staining for polymorphonuclear (PMN) leukocytes with antibodies against neutrophil elastase (Abcam ab68672). (C) Weak staining for P2Y₂R expression (H70 antibody: SC-20124), deficiency of P2Y₂R is agreement with low P2Y₂R (TaqMan measurement). (D) Weak staining for uPA is correlated to low macrophage expression.