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. 2018 Apr 23;62(9):1700941. doi: 10.1002/mnfr.201700941

Figure 4.

Figure 4

Feeding the diabetes mellitus (DM) mice with the RSV‐enriched diet increased mitochondrial content and improved aberrant muscle morphological features in the gastrocnemius (Gastroc) muscle. A) Succinate dehydrogenase (SDH) staining was performed on 10 μm‐thick sections from Gastroc muscles frozen in liquid nitrogen‐cooled isopentane. B) Quantification of succinate dehydrogenase (SDH) stain intensity (expressed in A.U.) and C) number of type I (slow oxidative), IIa (fast oxidative glycolytic) muscle fibers, and type IIb (fast glycolytic) muscle fibers. Scale bar: 100 μm. D) Transmission electron microscopy micrographs (magnification: 12 000×) of Gastroc muscles. White arrows indicate mitochondria. Black arrows indicate the Z‐line. Brackets identify the I‐bands. Scale bar: 1 μm. E) Quantification of mitochondrial amount (number per field) and F) size (average diameter, nm). Data are expressed as mean ± SD, n = 4 per group; ***p < 0.001 between control (CTL) and DM groups. #p < 0.05, ##p < 0.01, ###p < 0.001 between DM and DM+RSV groups.