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. 2018 Apr 23;62(9):1700941. doi: 10.1002/mnfr.201700941

Figure 5.

Figure 5

Feeding the diabetes mellitus (DM) mice with the RSV‐enriched diet improved mitochondrial biogenesis in the quadriceps muscle. A) Representative western blots using antibodies against nuclear respiratory factor (NRF‐1), ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide (ATP5B), cytochrome c oxidase subunit IV (Cox IV), peroxisome proliferator‐activated receptor‐γ coactivator 1‐α (PGC‐1α), and GAPDH. After quantification, the B) NRF‐1/GAPDH, C) ATP5B/GAPDH, D) Cox IV/GAPDH, and E) PGC‐1α/GAPDH ratios were calculated in mice fed either the control (CTL) or RSV‐enriched diet. GAPDH was used as a loading control. F) Representative western blots using antibodies against mitochondrial transcription factor A (mtTFA) and voltage‐dependent anion channel (VDAC), and the mtTFA/VDAC ratio was determined. The mitochondrial protein VDAC was used as a loading control. Data are expressed as fold change versus CTL and reported as mean ± SD, n = 4–6 per group; *p < 0.05; **p < 0.01 between CTL and DM groups. #p < 0.05 between DM and DM+RSV groups; †† p < 0.05 between CTL+RSV and DM+RSV groups.