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. 2018 Jan 16;57(23):3227–3236. doi: 10.1021/acs.biochem.7b01282

Figure 3.

Figure 3

Dependence of v/[E] (s–1) on HPO32– for the reduction of GA by NADH (0.2 mM) catalyzed by Q295 mutants of hlGPDH at pH 7.5 (10 mM TEA buffer), 25 °C, and I = 0.12 (NaCl) on the concentration of HPO32– for reactions at different fixed concentrations of GA. (A) Q295G mutant: ●, 3.6 mM GA; ▼, 3.0 mM; ◆, 2.4 mM; ○, 1.8 mM; ■, 1.2 mM; ▲, 0.6 mM. (B) Q295S: ●, 3.6 mM GA; ▼, 3.0 mM; ◆, 2.4 mM; ○, 1.8 mM; ■, 1.2 mM; ▲, 0.6 mM. (C) Q295A: ●, 3.6 mM GA; ◆, 2.4 mM; ■, 1.2 mM; ▲, 0.6 mM. (D) Q295N: ●, 3.6 mM GA; ◆, 2.4 mM; ■, 1.2 mM; ▲, 0.6 mM.