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Linear correlation, with slope of 1.1 ± 0.02, between the intrinsic phosphite dianion binding energy (ΔG⧧)HPi expressed at the transition state for the reduction of the truncated substrate glycolaldehyde by NADH, and the intrinsic phosphodianion binding energy (ΔG⧧)Pi expressed at the transition state for the reduction of DHAP by NADH catalyzed by wild-type and Q295 mutants of hlGPDH.
