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. 2018 Jan 3;73(7):845–852. doi: 10.1093/gerona/glx249

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© The Author(s) 2018. Published by Oxford University Press on behalf of The Gerontological Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Validation of interferon gamma (A) and (B) epithelial to mesenchymal transition pathways in the rat kidney. mRNA amounts of the selected genes were quantified by real-time quantitative polymerase chain reaction in the kidneys from 6-month-old rats treated with vehicle and 24-month-old rats treated with vehicle or RAD001. mRNA amounts of the genes of interest were normalized to a geometric mean of reference genes Cox7a2, Vps26a, and TATA-box-binding protein. Data are mean ± SD. **p ≤ .01; ***p ≤ .001; ****p ≤ .0001. Y-axes represent arbitrary units for normalized mRNA amounts. N = 8–10 rats per group.