Table 2. Regression and statistical parameters for the determination of DAS in rat plasma by the proposed UPLC-MS/MS method.
| Linearity range (ng/mL) | 1–500 |
|---|---|
| LLOQ (ng/mL) | 1.0 |
| LLOD (ng/mL) | 0.4 |
| Intercept (a) | -0.0240 |
| Slope (b) | 0.0060 |
| Correlation Coefficient (r) | 0.9998 |
| Sa | 0.0060 |
| Sb | 0.0005 |
| Sy/x | 0.0169 |
| F | 29265.89 |
| Significance F | 2.6914×10−12 |
LLOQ, lower limit of quantification; LLOD, lower limit of detection
Sa, standard deviation of intercept; Sb, standard deviation of slope
Sy/x, standard deviation of residuals; F: variance ratio equals the mean of squares due to regression divided by the mean of squares about regression (due to residuals).