Fig 4.

ALP activity (A) and immunolocalization (B) of hBM-MSCs cultured for 28 days onto TiO₂ with/without PEMF stimulation. A) ALP activity determined colorimetrically, corrected for the protein content measured with the BCA Protein Assay Kit and expressed as millimoles of p-nitrophenol produced per min per mg of protein. Bars express the mean values ± SEM of results from three measurements in two separated experiments (* p < 0.05). C). Immunolocalization of ALP following incubation with rabbit anti-human ALP primary antibody and detected with goat anti-rabbit secondary antibody (Alexa flour 488). Nuclei (in blue) were counterstained with Hoechst 33342. Magnification 40X; the scale bar represents 50 μm.